Enzyme Methyl sequençing of coral samples from different geographical regions under contrasted thermal regimes
This dataset contains libraries of 3 coral species: Acropora hyacinthus, Porites lobata and Poscillopora acuta. In three islands with contrasting thermal regimes, the three species were sampled and brought back to the laboratory to induce an experimental thermal stress. The different colonies were split into two conditions. One part was placed in tanks filled with seawater at a given control temperature, the other part in tanks where the water temperature was increased. The samples in this dataset correspond to part of the control condition samples from French Polynesia and New Caledonia.
Simple
- Title
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Enzyme Methyl sequençing of coral samples from different geographical regions under contrasted thermal regimes
- Date (Creation)
- 2023-11-07
- Date (Publication)
- 2024-01-19
- Citation identifier
- FR-330-715-368-00032-IFR_BIOINFO_SAVE_SEQ_DNA
- Abstract
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This dataset contains libraries of 3 coral species: Acropora hyacinthus, Porites lobata and Poscillopora acuta. In three islands with contrasting thermal regimes, the three species were sampled and brought back to the laboratory to induce an experimental thermal stress. The different colonies were split into two conditions. One part was placed in tanks filled with seawater at a given control temperature, the other part in tanks where the water temperature was increased. The samples in this dataset correspond to part of the control condition samples from French Polynesia and New Caledonia.
- Credit
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Plateforme INTEGRAGEN
- Point of contact
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Organisation name Individual name Electronic mail address Role IFREMER
Vidal Dupiol Jeremie
Author UPVD
Rey Olivier
Author IFREMER
Buso Pauline
Author UPVD
Toulza Eve
Author IFREMER
Romatif Océane
Author IFREMER
Pouzadoux Juliette
Author EPHE
Clerissi Camille
Author CCC
Bonito Victor
Author IRD
Rodolfo-Metalpa Riccardo
Author IRD
Houlbreque Fanny
Author Aquarium Monaco
Fouré Laurent
Author IFREMER
Mitta Guillaume
Author
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GEMET - INSPIRE themes, version 1.0
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Habitats and biotopes
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Thèmes Sextant
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/Biological Environment/Bioinformatics
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ODATIS aggregation parameters and Essential Variable names
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Bioinformatics
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- Use limitation
- CC-BY (Creative Commons - Attribution)
- Access constraints
- Restricted
- Use constraints
- Restricted
- Spatial representation type
- Grid
- Language
- English
- Character set
- UTF8
- Topic category
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- Oceans
- Reference system identifier
- EPSG / WGS 84 (EPSG:4326) / 8.6
- Distribution format
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Name Version
- OnLine resource
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Protocol Linkage Name NETWORK:LINK
/home/ref-bioinfo/ifremer/ihpe/coral-save
- Hierarchy level
- Dataset
- Statement
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Earth is experiencing environmental changes accelerating biodiversity erosion. Coral reefs are one of the most affected ecosystems by these changes because it relies on corals that leave at their upper thermal limit. Corals intraspecific diversity is crucial for their maintenance since it defines the adaptive potential of organisms in the face of current changes. Intraspecific diversity initially encompasses genetic, phenotypic and functional diversity within a species. However, non-genetically encoded elements such as the epigenetic information and the holobiont composition can vary between individuals and contribute to phenotypic adjustment. This project will combine sequencing technologies and innovative approaches to: i) better understand the spatial and temporal dynamics of intraspecific diversity in corals; ii) understand the genetic, epigenetic and microbial mechanisms of coral adaptation/acclimatization to thermal stress and iii) identify biomarkers of thermotolerance to enhance ecosystem management. These will be achieved by studying in space and time natural coral populations along an ecological gradient of thermal stress frequency and an integrative Omic approach.
The sequences of the 124 samples (control treatement) were carried out by the INTEGRAGEN platform. The samples of 3 species come from New Caledonia and French Polynesia.
Sequencing platform : NovaSeq
Control Software : 1.8.1
Primary Analysis RTA : 3.4.4
Type of sequencing : 2 * 150 bases
Index size : 17
Sequencing chemistry : 1.8.0
Mismatches : 0
With failed reads: no
Adapter sequence : none
Metadata
- File identifier
- 1f0f2c1d-f4af-4292-b530-da1ff94323d2
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Date stamp
- 2024-01-29T16:19:58.833Z
- Metadata standard name
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ISO 19115:2003/19139 - SEXTANT
- Metadata standard version
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1.0
- Metadata author
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Organisation name Individual name Electronic mail address Role IFREMER
Vidal Dupiol Jeremie
Local service desk