Title

Provigas: protection antivirale chez l'huitre crassostrea gigas par le poly(I:C)

Date (Creation)

2016-11-29

Citation identifier

FR-330-715-368-00032-IFR_BIOINFO_PROVIGAS

Citation identifier

DOI:10.12770/78b72405-f1ca-44a5-ae15-65916de3b2dd

Abstract

Données quantitatives d'expression de gènes acquises par approche RNAseq et séquençage Illumina chez l'huitre creuse (Crassostrea gigas) exposée ou non à une primo-exposition par le poly (I:C) et infectées par le virus OSHV-1 µVar

Credit

UMR5244 - Interaction Hotes Pathogenes Environnement - Projet région Occitanie PROVIGAS

Point of contact

Organisation name	Individual name	Electronic mail address	Role
IFREMER	Maxime Lafont	maxime.lafont@hotmail.fr	Author
IFREMER	Montagnani Caroline	Caroline.Montagnani@ifremer.fr	Author
IFREMER		sismer@ifremer.fr	Publisher

GEMET - INSPIRE themes, version 1.0

• Habitats et biotopes

Thèmes Sextant

• /Milieu biologique/Bio-Informatique

ODATIS aggregation parameters and Essential Variable names

• Bioinformatique

Use limitation

Donnée en accès restreint

Access constraints

Restricted

Use constraints

Restricted

Language

Français

Character set

UTF8

Topic category

• Environment

Reference system identifier

EPSG / WGS 84 (EPSG:4326) / 8.6

Distribution format

Name	Version

OnLine resource

Protocol	Linkage	Name
DOI	https://doi.org/10.12770/78b72405-f1ca-44a5-ae15-65916de3b2dd	DOI du jeu de donnée
NETWORK:LINK	/home/ref-bioinfo/ifremer/ihpe/provigas

Hierarchy level

Dataset

Statement

Quality check of total RNA and total RNA integrity was analyzed by capillary electrophoresis (Agilent BioAnalyzer 2100) showing no degradation. mRNAs purification and library construction was performed by MGX (Montpellier Genomix, Institut de Génomique fonctionnelle, Montpellier, France) using the Illumina TruSeq Stranded mRNA Sample Preparation kit according to the manufacturer’s protocol. Libraries were sequenced by MGX in single-end, 50 bp length fragments, using an Illumina HiSeq 2500. All Illumina sequenced reads were aligned to the C. gigas V9 reference genome (Zhang et al. 2012) and 73.9% of reads on average mapped on this genome

Metadata

File identifier

78b72405-f1ca-44a5-ae15-65916de3b2dd

Metadata language

Français

Character set

UTF8

Hierarchy level

Dataset

Date stamp

2025-01-24T15:56:32.814055Z

Metadata standard name

ISO 19115:2003/19139 - SEXTANT

Metadata standard version

1.0

Metadata author

Organisation name	Individual name	Electronic mail address	Role
IFREMER	Montagnani Caroline	Caroline.Montagnani@ifremer.fr	Local service desk