Title

Monitoring bacterial diversity in intertidal mudflats

Date (Creation)

2006-01-01T00:00:00

Citation identifier

FR-DCSMM-PDS-VASIREMI

Presentation form

Digital profile

Abstract

Intertidal mudflats are a key system of the network, being closely connected to ecosystems which form the transition between the watershed area and the ocean. Their high productivity is linked to activity within a diatom biofilm, the functioning and future of which have recently been the subject of many research programmes. However, the determinism and future of bacterial production associated with the secretion of ExoPolySaccharides within the biofilm is largely unknown.

Early studies have nevertheless shown that this production is at least as high as that of diatoms. An integrated view of the ecological functioning of intertidal mudflats within a more global schema of carbon flow therefore needs to define and quantify the determinism of this bacterial production and it future within the ecosystem.

Credit

Data producer : UMR LIENSS 7276

Status

On going

Point of contact

Organisation name	Individual name	Electronic mail address	Role
UMR LIENSS 7276	Dupuy Christine	cdupuy@univ-lr.fr	Local service desk

Maintenance and update frequency

Unknown

Maintenance note

Network duration : temporary network

Theme

• Diversity

• Abundance

• Bacteria

• Mudflats

• Intertidal

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Sous-regions marines

• Bay of Biscay

DCSMM : Type d'espace concerné

• Pas d'espace particulier concerné

DCSMM : Descripteurs

• D4: Marine Food Webs

• D1: Biodiversity

DCSMM : Méthodes de recueil des données

• Observation par point

Thèmes Sextant

• /Biological Environment/Habitats

Access constraints

Other restrictions

Other legal constraints

Confidential access

Spatial representation type

Vector

Denominator

50000

Language

Français

Character set

UTF8

Topic category

• Oceans

Supplemental Information

Prime contractor :

UMR LIENSS 7276 2007-2013

Contracting Authorities :

UMR LIENSS 7276 2007-2013

Backers :

ANR 2007-2011

CPER 2011-2013

Reference system identifier

EPSG / WGS 84 (EPSG:4326) / 7.9

Topology level

Geometry only

Geometric object type

Complex

Geometric object count

0

Hierarchy level

Dataset

Statement

Within the sediment :

- Bacterial diversity obtained through pyrosequencing (outsourced).

- Total abundance of prokaryotes measured using flow cytometry (methodology currently in the development phase).

- Quantification of bacterial denitrification obtained using quantitative PCR of the genes coding the enzymes involved in the denitrifying function.

- Bacterial production measured using radioactive markers.

On the mudflats, as the temporal variability of the diversity/abundance of prokaryotes is very high (unlike that of spatial variability), samples must be repeated over 7 days in order to have a sample representative of a given point.

Description

Data type :

Prokaryota

Measured or observed parameters :

Bacterial abundance

Bacterial diverstiy

Quantification of bacterial denitrification

Parameters resolution :

3 replicates over an area of around 100 x 100m, at 1.5 km off from the coast

Fréquence d'observation :

1 sample daily for 7 days, 4 times a year (seasonal)

Description

Sampling method : Spatial

Metadata

File identifier

a6c916f3-56d1-42e7-9e68-0ab3b5800d52

Metadata language

Français

Other language

Language	Character encoding
English

Character set

UTF8

Hierarchy level

Series

Date stamp

2020-06-04T00:24:40

Metadata standard name

ISO 19115:2003/19139 - SEXTANT

Metadata standard version

1.0

Metadata author

Organisation name	Individual name	Electronic mail address	Role
Sextant		sextant@ifremer.fr	Local service desk