Title

DNA sequencing of Crassostrea gigas Pacific oyster spat experimentally infected with OsHV-1 virus

Date (Creation)

2023-01-03

Date (Publication)

2024-01-29

Citation identifier

FR-330-715-368-00032-IFR_BIOINFO_2022_WGS_OsHV1-infected-Cgigas_20230106_ASIM_MM

Abstract

DNA sequencing of Crassostrea gigas Pacific oyster spat experimentally infected with OsHV-1 virus from oyster basin of Marennes-Oleron

Credit

IFREMER (RBE/IHPE and RBE/ASIM)

Point of contact

Organisation name	Individual name	Electronic mail address	Role
IFREMER		sismer@ifremer.fr	Publisher
IFREMER	Mege Mickael	mickael.mege@ifremer.fr	Author
IFREMER	Pelletier Camille	Camille.Pelletier@ifremer.fr	Author
IFREMER	Morga Benjamin	Benjamin.Morga@ifremer.fr	Author
IFREMER	Vidal Dupiol Jeremie	Jeremie.Vidal.Dupiol@ifremer.fr	Author

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Thèmes Sextant

• /Biological Environment/Bioinformatics

ODATIS aggregation parameters and Essential Variable names

• Bioinformatics

Use limitation

CC-BY-NC (Creative Commons - Attribution, No commercial usage)

Access constraints

License

Use constraints

License

Spatial representation type

Grid

Language

English

Character set

UTF8

Topic category

• Oceans

Reference system identifier

EPSG / WGS 84 (EPSG:4326) / 8.6

Distribution format

Name	Version

OnLine resource

Protocol	Linkage	Name
NETWORK:LINK	/home/ref-bioinfo/ifremer/asim/2022_WGS_OsHV1-infected-Cgigas

Hierarchy level

Dataset

Statement

C. gigas genitors were sampled in breeding or non-breeding areas from Brittany, Marennes oleron Bay, Arcachon bay and Thau lagoon. Heigt families of C. gigas spat were produced in La Tremblade hatchery. These spat were experimentally infected with OsHV-1 virus from oyster of Marennes-Oleron bay. Moribund oysters and contaminated seawaters between post-infection were sampled. The DNA was extracted (Magattract kit for oyster/QIAamp DNA Mini Kit for seawater) and transfert to the Integragen sequencing platform, all samples were sequenced using Illumina sequencing technology (NovaSeq 6000 PCR free).

Metadata

File identifier

d01bb4d8-67b2-4be6-84de-18aeb5b2a24a

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Date stamp

2024-01-29T13:32:12.578Z

Metadata standard name

ISO 19115:2003/19139 - SEXTANT

Metadata standard version

1.0

Metadata author

Organisation name	Individual name	Electronic mail address	Role
IFREMER	Mege Mickael	mickael.mege@ifremer.fr	Local service desk