Metagenomic analyses of the hydrothermal shrimp Rimicaris exoculata cephalothoracic epibiotic community
We use shotgun metagenomics to reconstruct novel Rimicaris exoculata cephalothoracic epibiotic genomes and unravel their potential metabolic functions
Data access Chemin d'accès |
/home/ref-bioinfo/ifremer/lmee/REX_Reveillaud
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Date(s) Date(s) |
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Author(s) Auteur(s) |
Aube Johanne
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IFREMER
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Cambon-Bonavita Marie-Anne ( IFREMER ) |
Contact(s) Contact(s) |
IFREMER
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Source Source |
Ifremer - Laboratoire de Microbiologie des Environnements Extrêmes - Projets ABYSS, REMIMA and LabexMer |
Lineage Généalogie |
We collected Rimicaris exoculata specimens using a suction sampler manipulated by the Remote Operated Vehicle (ROV) Victor 6000 on board on the research vessel Pourquoi pas?. Samples were taken from three hydrothermal sites on the Mid Atlantic Ridge: Rainbow during the MoMAR and the Biobaz cruise, TAG and Snake Pit during the BICOSE cruise. We performed DNA extraction of the six Rimicaris cephalothoracic appendages (2 branchiostegites + 2 scaphognathites + 2 exopodites). We sheared DNA to 175 bp using a Covaris S-series sonicator and completed metagenomic library construction using the Ovation Ultralow Library DR multiplex system (Nugen) following manufacturer’s instructions. We performed metagenomic sequencing on an Illumina HiSeq 1000 and a NextSeq at the W.M. Keck sequencing facility at the Marine Biological Laboratory. All libraries were paired-end, with a 30 bp overlap, resulting in an average merged read length of 170 bp. |
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Spatial informations Informations géographiques |
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Citation proposal Proposition de citation
Aube Johanne , Cambon-Bonavita Marie-Anne (2018).Metagenomic analyses of the hydrothermal shrimp Rimicaris exoculata cephalothoracic epibiotic community.IFREMER
http://dx.doi.org/10.12770/4186eeef-32b1-4ffb-9a40-61a02a3852b7
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