Provigas: protection antivirale chez l'huitre crassostrea gigas par le poly(I:C)
Données quantitatives d'expression de gènes acquises par approche RNAseq et séquençage Illumina chez l'huitre creuse (Crassostrea gigas) exposée ou non à une primo-exposition par le poly (I:C) et infectées par le virus OSHV-1 µVar
Data access Chemin d'accès |
/home/ref-bioinfo/ifremer/ihpe/provigas
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Date(s) Date(s) |
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Author(s) Auteur(s) |
Maxime Lafont
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IFREMER
)
Montagnani Caroline ( IFREMER ) |
Contact(s) Contact(s) |
IFREMER
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Source Source |
UMR5244 - Interaction Hotes Pathogenes Environnement - Projet région Occitanie PROVIGAS |
Lineage Généalogie |
Quality check of total RNA and total RNA integrity was analyzed by capillary electrophoresis (Agilent BioAnalyzer 2100) showing no degradation. mRNAs purification and library construction was performed by MGX (Montpellier Genomix, Institut de Génomique fonctionnelle, Montpellier, France) using the Illumina TruSeq Stranded mRNA Sample Preparation kit according to the manufacturer’s protocol. Libraries were sequenced by MGX in single-end, 50 bp length fragments, using an Illumina HiSeq 2500. All Illumina sequenced reads were aligned to the C. gigas V9 reference genome (Zhang et al. 2012) and 73.9% of reads on average mapped on this genome |
Constraints Contraintes |
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Spatial informations Informations géographiques |
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Citation proposal Proposition de citation
Maxime Lafont , Montagnani Caroline .Provigas: protection antivirale chez l'huitre crassostrea gigas par le poly(I:C).IFREMER
http://dx.doi.org/10.12770/78b72405-f1ca-44a5-ae15-65916de3b2dd