Crassostrea gigas hemocytes scRNA-seq sequences
scRNA-seq reads from a Pacific oyster (Crassostrea gigas) hemocyte preparation. Hemocytes were isolated from a unique immunologically naive animal (Ifremer Standardized Animal, 18 months) and single-cell drop-seq technology was applied to 3,000 individual hemocytes.
Data accessChemin d'accès | /home/ref-bioinfo-public/ifremer/ihpe/hemofight |
Date(s)Date(s) |
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Author(s)Auteur(s) | Vignal Emmanuel
(University of Montpellier (IHPE UMR5244))
De la Forest Divonne Sébastien (University of Perpignan (IHPE UMR5244)) Charrière Guillaume (University of Montpellier (IHPE UMR5244)) Destoumieux-Garzon Delphine (CNRS (IHPE UMR5244)) Gourbal Benjamin (University of Perpignan (IHPE UMR5244)) Mitta Guillaume (IFREMER (Institut Louis-Malardé, Univ Polynésie française)) Courtay Gaelle (IFREMER (IHPE UMR5244)) Romatif Océane (IFREMER (IHPE UMR5244)) Pouzadoux Juliette (IFREMER (IHPE UMR5244)) |
Contact(s)Contact(s) | IFREMER
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SourceSource |
IHPE UMR5244 |
LineageGénéalogie |
The 10X Genomics protocol was followed to prepare gel in emulsion beads containing single cells, hydrogel beads, and reverse transcription reagents, perform barcoded cDNA synthesis, and generate sequencing libraries from pooled cDNAs. The concentration of single cell suspension was approximately 1000 cells / μL, and cells were loaded according to the 10X protocol to capture approximately 3000 cells per reaction. Library construction was performed using 10X reagents according to the manufacturer. Libraries (paired end reads 75 bp) were sequenced on an Illumina NovaSeq using two sequencing lane per sample. |
ConstraintsContraintes |
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Spatial informationsInformations géographiques |
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Citation proposalProposition de citation
Vignal Emmanuel, De la Forest Divonne Sébastien, Charrière Guillaume, Destoumieux-Garzon Delphine, Gourbal Benjamin, Mitta Guillaume, Courtay Gaelle, Romatif Océane, Pouzadoux Juliette (2024).Crassostrea gigas hemocytes scRNA-seq sequences.IFREMER
https://doi.org/10.12770/ee5fd99f-d88f-4c6c-9b77-bfe1c88ca86f